Hotspot

psbA-trnK-UUU

LSC · 1463-1719 · 257 bp

Back to Sophora

Pi 0.0366

MarkerSeek score 74.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 32
pi: 0.0366
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GCTCTCGCGTCTCTCTAAAA	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	57.5	58.8	281-326 bp	1.0	81.0
psbA-trnK-UUU_p2	GCTCTCGCGTCTCTCTAAAA	GACTAGTTCCGGGTTCGAGT	50.0	55.0	57.5	58.8	282-327 bp	1.0	81.0
psbA-trnK-UUU_p3	GCTCTCGCGTCTCTCTAAAA	ATCCGACTAGTTCCGGGTTC	50.0	55.0	57.5	58.9	286-331 bp	1.0	80.5
psbA-trnK-UUU_p4	TACAGAAGCGACCCCATAGG	ACTAGTTCCGGGTTCGAGTC	55.0	55.0	58.6	58.8	306-351 bp	1.0	80.3
psbA-trnK-UUU_p5	TACAGAAGCGACCCCATAGG	GACTAGTTCCGGGTTCGAGT	55.0	55.0	58.6	58.8	307-352 bp	1.0	80.2