Hotspot

trnH-GUG-psbA

LSC · 129-400 · 272 bp

Back to Sophora

Pi 0.0883
MarkerSeek score 73.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
272
variable_sites
68
pi
0.0883
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCGAGGCTCCATCTATAAATGG 55.0 45.5 58.1 57.2 245-350 bp 1.0 68.5
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TCGAGGCTCCATCTATAAATGG 50.0 45.5 57.9 57.2 259-364 bp 1.0 68.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TCGAGGCTCCATCTATAAATGG 50.0 45.5 57.9 57.2 258-363 bp 1.0 68.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TCGAGGCTCCATCTATAAATGG 50.0 45.5 57.9 57.2 260-365 bp 1.0 68.5
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC TCGAGGCTCCATCTATAAATGG 50.0 45.5 57.5 57.2 274-379 bp 1.0 65.7