Hotspot

petB

LSC · 78043-79482 · 1440 bp

Back to Sonneratia

Pi 0.00139

MarkerSeek score 52.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 5
pi: 0.00139
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTGGTAGTTCGATCGTGGAA	TGCTATTGTTCCTCCTCAAGTG	45.0	45.5	56.6	58.1	1543-1546 bp	1.0	52.7
petB_p2	TTGGTAGTTCGATCGTGGAA	GCTATTGTTCCTCCTCAAGTGA	45.0	45.5	56.6	57.8	1542-1545 bp	1.0	51.5
petB_p3	ATTGGTAGTTCGATCGTGGA	TGCTATTGTTCCTCCTCAAGTG	45.0	45.5	56.4	58.1	1544-1547 bp	1.0	50.9
petB_p4	TGGTAGTTCGATCGTGGAAT	TGCTATTGTTCCTCCTCAAGTG	45.0	45.5	56.4	58.1	1542-1545 bp	1.0	50.9
petB_p5	TTGGTAGTTCGATCGTGGAA	TGCTATTGTTCCTCCTCAAGT	45.0	42.9	56.6	56.6	1543-1546 bp	1.0	50.3