Hotspot

rpl32-trnL-UAG

SSC · 114069-114742 · 674 bp

Back to Sonneratia

Pi 0.00772
MarkerSeek score 54.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
674
variable_sites
14
pi
0.00772
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCGAAGTCTCTTTCAACCG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 518-776 bp 1.0 83.7
rpl32-trnL-UAG_p2 AGCGAAGTCTCTTTCAACCG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 519-777 bp 1.0 83.7
rpl32-trnL-UAG_p3 AGCGAAGTCTCTTTCAACCG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 528-786 bp 1.0 82.8
rpl32-trnL-UAG_p4 AGCGAAGTCTCTTTCAACCG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.2 57.8 527-785 bp 1.0 82.2
rpl32-trnL-UAG_p5 AGCGAAGTCTCTTTCAACCG CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.2 57.8 526-784 bp 1.0 82.2