Hotspot

rpl33-rps18

LSC · 70836-71144 · 309 bp

Back to Sonneratia

Pi 0.0305

MarkerSeek score 67.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 33
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACATACAATTCACGGGGAGA	TCAATTCGATCCCCCGATTG	45.0	50.0	56.2	57.7	426-429 bp	1.0	72.1
rpl33-rps18_p2	AACATACAATTCACGGGGAGA	TCAATTCGATCCCCCGATTG	42.9	50.0	57.0	57.7	427-430 bp	1.0	70.3
rpl33-rps18_p3	ACATACAATTCACGGGGAGA	ATTCGATCCCCCGATTGGAT	45.0	50.0	56.2	58.6	423-426 bp	1.0	69.1
rpl33-rps18_p4	ACAAACATACAATTCACGGGG	TCAATTCGATCCCCCGATTG	42.9	50.0	56.8	57.7	430-433 bp	1.0	68.5
rpl33-rps18_p5	ACATACAATTCACGGGGAGAT	TCAATTCGATCCCCCGATTG	42.9	50.0	56.8	57.7	426-429 bp	1.0	68.5