Hotspot

ndhC-trnV-UAC

LSC · 52629-54382 · 1754 bp

Back to Sonneratia

Pi 0.00179

MarkerSeek score 51.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1754
variable_sites: 8
pi: 0.00179
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	ACATCACAAACACAAGGGCT	50.0	45.0	57.9	57.9	1904-1908 bp	1.0	78.6
ndhC-trnV-UAC_p2	AATTGGGGCTAAAACTCCGG	ACATCACAAACACAAGGGCT	50.0	45.0	58.2	57.9	1908-1912 bp	1.0	78.5
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	CACATCACAAACACAAGGGC	50.0	50.0	57.9	57.9	1905-1909 bp	1.0	78.1
ndhC-trnV-UAC_p4	AATTGGGGCTAAAACTCCGG	CACATCACAAACACAAGGGC	50.0	50.0	58.2	57.9	1909-1913 bp	1.0	78.0
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	AGGGCTATAGCTCAGTTGGT	50.0	50.0	57.9	57.8	1890-1894 bp	1.0	77.8