Hotspot

trnF-GAA-ndhJ

LSC · 50177-50939 · 763 bp

Back to Sonneratia

Pi 0.00262

MarkerSeek score 53.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 7
pi: 0.00262
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	907-912 bp	1.0	79.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	871-876 bp	1.0	79.4
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATAGGCTGGCCCTTACGTAA	55.0	50.0	58.0	58.2	897-902 bp	1.0	79.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	861-866 bp	1.0	78.8
trnF-GAA-ndhJ_p5	TGCCTAAGTAATGGTCGGGA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	923-928 bp	1.0	78.7