Hotspot

trnC-GCA-petN

LSC · 28318-29195 · 878 bp

Back to Sonneratia

Pi 0.0026

MarkerSeek score 58.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 6
pi: 0.0026
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	867-950 bp	1.0	83.8
trnC-GCA-petN_p2	GGTATCATTTTGGCGGCATG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.9	58.0	920-1003 bp	1.0	83.3
trnC-GCA-petN_p3	GTATCATTTTGGCGGCATGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.9	58.0	919-1002 bp	1.0	83.3
trnC-GCA-petN_p4	GGGTGTCGCCTGATTAACAA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.2	58.0	849-932 bp	1.0	83.0
trnC-GCA-petN_p5	TGGTATCATTTTGGCGGCAT	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	58.2	58.0	921-1004 bp	1.0	82.6