Hotspot

trnG-GCC-trnR-UCU

LSC · 9972-10282 · 311 bp

Back to Sonneratia

Pi 0.0101

MarkerSeek score 42.40

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 311
variable_sites: 8
pi: 0.0101
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TCAAAAATGCGAAAAAGCGGA	CAGGAACAGAAGGAACGCTT	38.1	50.0	57.9	58.1	463 bp	0.142857	36.1
trnG-GCC-trnR-UCU_p2	TCAAAAATGCGAAAAAGCGGA	TTCAGGAACAGAAGGAACGC	38.1	50.0	57.9	58.1	465 bp	0.142857	36.1
trnG-GCC-trnR-UCU_p3	CTCAAAAATGCGAAAAAGCGG	CAGGAACAGAAGGAACGCTT	42.9	50.0	57.6	58.1	464 bp	0.142857	33.4
trnG-GCC-trnR-UCU_p4	CTCAAAAATGCGAAAAAGCGG	TTCAGGAACAGAAGGAACGC	42.9	50.0	57.6	58.1	466 bp	0.142857	33.4
trnG-GCC-trnR-UCU_p5	CAAAAATGCGAAAAAGCGGA	CAGGAACAGAAGGAACGCTT	40.0	50.0	56.4	58.1	462 bp	0.142857	32.2