Hotspot

rps15

SSC · 124276-124539 · 264 bp

Back to Sonerila

Pi 0.0125

MarkerSeek score 44.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 14
pi: 0.0125
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGCATTGATGGGTGATGAGG	AACGGGGGTTGTACACATTC	50.0	50.0	57.9	58.1	735-740 bp	0.2	52.8
rps15_p2	GCATTGATGGGTGATGAGGT	AACGGGGGTTGTACACATTC	50.0	50.0	57.9	58.1	734-739 bp	0.2	52.8
rps15_p3	TAACTGTTCGGTTTTCGGCA	AACGGGGGTTGTACACATTC	45.0	50.0	58.0	58.1	848-853 bp	0.2	52.2
rps15_p4	TCTGTGCACCAATATCTGCC	AACGGGGGTTGTACACATTC	50.0	50.0	58.0	58.1	1052-1057 bp	0.2	51.5
rps15_p5	TCTGTGCACCAATATCTGCC	AAAAACGGGGGTTGTACACA	50.0	45.0	58.0	57.9	1055-1060 bp	0.2	51.1