Hotspot

ndhD-psaC

SSC · 118124-118267 · 144 bp

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Pi 0.0511

MarkerSeek score 81.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 144
variable_sites: 20
pi: 0.0511
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCAAGGAAAATGATTCGT	AAACAACTCGAAGCATGGGT	35.0	45.0	53.4	58.0	192-199 bp	1.0	54.7
ndhD-psaC_p2	TGTTAACCAAGGAAAATGATTCGT	AAACAACTCGAAGCATGGGT	33.3	45.0	57.4	58.0	196-203 bp	1.0	54.7
ndhD-psaC_p3	AACCAAGGAAAATGATTCGT	GGCATGAAACAACTCGAAGC	35.0	50.0	53.4	58.0	198-205 bp	1.0	54.6
ndhD-psaC_p4	TGTTAACCAAGGAAAATGATTCGT	GGCATGAAACAACTCGAAGC	33.3	50.0	57.4	58.0	202-209 bp	1.0	54.6
ndhD-psaC_p5	AACCAAGGAAAATGATTCGT	AGAGATGTGAATCCGCTTGC	35.0	50.0	53.4	58.3	252-259 bp	1.0	50.8