Hotspot

ndhF-rpl32

SSC · 114157-114723 · 567 bp

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Pi 0.0425

MarkerSeek score 77.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 567
variable_sites: 71
pi: 0.0425
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAATAGGGATATGTATAGTCCAT	AGGCTTTTATTTTCCAAATATTCT	34.8	25.0	52.1	53.2	486-663 bp	1.0	52.0
ndhF-rpl32_p2	GAATAGGGATATGTATAGTCCAT	TGTAAAATAGGCTTTTATTTTCCA	34.8	25.0	52.1	53.2	494-671 bp	1.0	52.0
ndhF-rpl32_p3	GAATAGGGATATGTATAGTCCAT	GGCTTTTATTTTCCAAATATTCTT	34.8	25.0	52.1	52.6	485-662 bp	1.0	52.0
ndhF-rpl32_p4	GAATAGGGATATGTATAGTCCAT	GGCTTTTATTTTCCAAATATTCTTACG	34.8	29.6	52.1	56.4	485-662 bp	0.9	48.0
ndhF-rpl32_p5	GAATAGGGATATGTATAGTCCAT	GCTTTTATTTTCCAAATATTCTTACGA	34.8	25.9	52.1	55.6	484-661 bp	0.9	48.0