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rps8-rpl14

LSC · 81387-81690 · 304 bp

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Pi 0.0641

MarkerSeek score 81.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 304
variable_sites: 57
pi: 0.0641
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GTCAGCAATAGTGTCTCTACCC	GAATTGAGACAGTTAAATTTCACT	50.0	29.2	58.0	53.8	383-401 bp	0.9	48.0
rps8-rpl14_p2	CAGCAATAGTGTCTCTACCCA	GAATTGAGACAGTTAAATTTCACT	47.6	29.2	56.8	53.8	381-399 bp	0.9	48.0
rps8-rpl14_p3	TCAGCAATAGTGTCTCTACCCA	GAATTGAGACAGTTAAATTTCACT	45.5	29.2	58.3	53.8	382-400 bp	0.9	48.0
rps8-rpl14_p4	GTCAGCAATAGTGTCTCTACCC	AATTGAGACAGTTAAATTTCACT	50.0	26.1	58.0	52.6	382-400 bp	0.9	48.0
rps8-rpl14_p5	CAGCAATAGTGTCTCTACCCA	AATTGAGACAGTTAAATTTCACT	47.6	26.1	56.8	52.6	380-398 bp	0.9	48.0