Hotspot

psbE-petL

LSC · 65077-66212 · 1136 bp

Back to Sonerila

Pi 0.0309

MarkerSeek score 82.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 120
pi: 0.0309
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGTAGAAAACCA	50.0	40.0	57.8	54.3	1194-1209 bp	1.0	61.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCAA	50.0	40.0	57.8	54.3	1193-1208 bp	1.0	61.0
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	57.8	57.6	1214-1225 bp	0.9	56.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GCTGAGGTTATAGTTAAAGCTGC	55.0	43.5	58.2	57.6	1209-1220 bp	0.9	56.5
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.4	57.6	1207-1218 bp	0.9	55.3