Hotspot

petA-psbJ

LSC · 63372-64269 · 898 bp

Back to Sonerila

Pi 0.0355

MarkerSeek score 80.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 110
pi: 0.0355
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGGTTCAATTATCCGAAATGA	ATACTCAGGGTTGGGCTCAT	33.3	50.0	52.9	58.1	890-972 bp	1.0	52.0
petA-psbJ_p2	AAGGTTCAATTATCCGAAATGA	ATACTCAGGGTTGGGCTCAT	31.8	50.0	53.8	58.1	891-973 bp	1.0	52.0
petA-psbJ_p3	AGGTTCAATTATCCGAAATGAA	ATACTCAGGGTTGGGCTCAT	31.8	50.0	53.8	58.1	890-972 bp	1.0	52.0
petA-psbJ_p4	AGGTTCAATTATCCGAAATGA	GTTCATACTCAGGGTTGGGC	33.3	55.0	52.9	58.2	894-976 bp	1.0	52.0
petA-psbJ_p5	AGGTTCAATTATCCGAAATGA	TTCATACTCAGGGTTGGGCT	33.3	50.0	52.9	58.3	893-975 bp	1.0	52.0