Hotspot

rbcL-accD

LSC · 57216-57738 · 523 bp

Back to Sonerila

Pi 0.0448

MarkerSeek score 83.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 59
pi: 0.0448
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	594-620 bp	1.0	88.7
rbcL-accD_p2	CTGCTGCTTGTGAGGTATGG	ACATCGAATTAAACCGCCGT	55.0	45.0	58.6	58.3	596-622 bp	1.0	84.6
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	AACATCGAATTAAACCGCCG	50.0	45.0	57.9	57.2	595-621 bp	1.0	84.3
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	CATCGAATTAAACCGCCGTT	50.0	45.0	57.9	57.2	593-619 bp	1.0	84.3
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	CATCGAATTAAACCGCCGTTT	50.0	42.9	57.9	57.9	593-619 bp	1.0	81.8