Hotspot

trnD-GUC-trnY-GUA

LSC · 30624-31078 · 455 bp

Back to Sonerila

Pi 0.035

MarkerSeek score 82.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 48
pi: 0.035
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TTTTATTCCGTCGGGACTGG	TGGCAATATGTCTACGCTGG	50.0	50.0	57.6	57.8	580 bp	0.1	39.0
trnD-GUC-trnY-GUA_p2	TTTTATTCCGTCGGGACTGG	GGCAATATGTCTACGCTGGT	50.0	50.0	57.6	57.8	579 bp	0.1	39.0
trnD-GUC-trnY-GUA_p3	TTTTATTCCGTCGGGACTGG	GAATGGCTTCTTCCTGGGTC	50.0	55.0	57.6	58.2	639 bp	0.1	38.9
trnD-GUC-trnY-GUA_p4	TTTTATTCCGTCGGGACTGG	GGGGACGGACTGTAAATTCG	50.0	55.0	57.6	58.4	601 bp	0.1	38.1
trnD-GUC-trnY-GUA_p5	TTTTATTCCGTCGGGACTGG	GGGACGGACTGTAAATTCGT	50.0	50.0	57.6	57.6	600 bp	0.1	37.9