Hotspot

psbM-trnD-GUC

LSC · 29851-30549 · 699 bp

Back to Sonerila

Pi 0.0279

MarkerSeek score 79.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 67
pi: 0.0279
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACTTTGACTCACGGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.4	58.6	689-888 bp	1.0	65.8
psbM-trnD-GUC_p2	TCACTTTGACTCACGGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.2	58.6	689-888 bp	1.0	64.1
psbM-trnD-GUC_p3	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	635-834 bp	1.0	64.0
psbM-trnD-GUC_p4	AATCACTTTGACTCACGGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	691-890 bp	1.0	63.9
psbM-trnD-GUC_p5	ATCACTTTGACTCACGGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	690-889 bp	1.0	63.9