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trnC-GCA-petN

LSC · 28484-29317 · 834 bp

Back to Sonerila

Pi 0.0287

MarkerSeek score 83.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 90
pi: 0.0287
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	874-919 bp	1.0	91.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCTTTAGAGTCCACTTCGCC	50.0	55.0	58.1	58.0	933-978 bp	0.9	86.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CTTTAGAGTCCACTTCGCCC	50.0	55.0	58.1	58.0	932-977 bp	0.9	86.9
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	868-913 bp	0.9	85.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GTGGTGCCTTTAGAGTCCAC	50.0	55.0	58.1	58.2	939-984 bp	0.9	85.2