Hotspot

ndhD-ccsA

Genome · 121653-121915 · 263 bp

Back to Sonchus

Pi 0.0209

MarkerSeek score 63.40

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: Genome
length_bp: 263
variable_sites: 19
pi: 0.0209
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCGATCGCCATTTTTCTACCT	TGGCGACTCTAGGCTTTCTT	42.9	50.0	57.5	58.7	471-492 bp	1.0	67.2
ndhD-ccsA_p2	CGATCGCCATTTTTCTACCTG	TGGCGACTCTAGGCTTTCTT	47.6	50.0	57.4	58.7	470-491 bp	1.0	66.8
ndhD-ccsA_p3	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCTT	42.9	50.0	57.4	58.7	408-429 bp	1.0	66.7
ndhD-ccsA_p4	TCGATCGCCATTTTTCTACCT	CAATTGTGGCGACTCTAGGC	42.9	55.0	57.5	59.0	477-498 bp	1.0	65.1
ndhD-ccsA_p5	TCGATCGCCATTTTTCTACCT	GCAATTGTGGCGACTCTAGG	42.9	55.0	57.5	59.0	478-499 bp	0.941176	62.2