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ndhC-HRV17_pgt018

Genome · 50250-51464 · 1215 bp

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Pi 0.00581

MarkerSeek score 60.10

Primer available yes

Label ndhC-HRV17_pgt018

Feature Metadata

label_name: ndhC-HRV17_pgt018
feature_type: igs
region: Genome
length_bp: 1215
variable_sites: 36
pi: 0.00581
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-HRV17_pgt018_p1	TCCTTTGCTACTCGGAGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1334-1396 bp	1.0	83.9
ndhC-HRV17_pgt018_p2	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1325-1387 bp	1.0	82.6
ndhC-HRV17_pgt018_p3	TTCTCCGGTCCTTTGCTACT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	1342-1404 bp	1.0	82.1
ndhC-HRV17_pgt018_p4	TTTCTCCGGTCCTTTGCTAC	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.5	58.1	1343-1405 bp	1.0	81.3
ndhC-HRV17_pgt018_p5	CTCGGAGCTAAAAATCCCGA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1324-1386 bp	1.0	80.3