Hotspot

HRV17_pgt017-ndhJ

Genome · 47988-48577 · 590 bp

Back to Sonchus

Pi 0.0052

MarkerSeek score 60.90

Primer available yes

Label HRV17_pgt017-ndhJ

Feature Metadata

label_name: HRV17_pgt017-ndhJ
feature_type: igs
region: Genome
length_bp: 590
variable_sites: 18
pi: 0.0052
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
HRV17_pgt017-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	507-734 bp	1.0	75.9
HRV17_pgt017-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	506-733 bp	1.0	75.9
HRV17_pgt017-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	514-741 bp	1.0	75.3
HRV17_pgt017-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	50.0	47.6	58.1	57.5	508-735 bp	1.0	73.8
HRV17_pgt017-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	50.0	47.6	58.1	58.6	507-734 bp	1.0	72.9