Hotspot

ycf3-HRV17_pgt014

Genome · 44113-45032 · 920 bp

Back to Sonchus

Pi 0.00671

MarkerSeek score 57.70

Primer available yes

Label ycf3-HRV17_pgt014

Feature Metadata

label_name: ycf3-HRV17_pgt014
feature_type: igs
region: Genome
length_bp: 920
variable_sites: 27
pi: 0.00671
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-HRV17_pgt014_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	967-1036 bp	1.0	76.6
ycf3-HRV17_pgt014_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	917-986 bp	1.0	76.3
ycf3-HRV17_pgt014_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	955-1024 bp	1.0	76.0
ycf3-HRV17_pgt014_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	919-988 bp	1.0	75.8
ycf3-HRV17_pgt014_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	945-1014 bp	1.0	74.7