Hotspot

HRV17_pgt008-rpoB

Genome · 12000-12914 · 915 bp

Back to Sonchus

Pi 0.00697

MarkerSeek score 57.60

Primer available yes

Label HRV17_pgt008-rpoB

Feature Metadata

label_name: HRV17_pgt008-rpoB
feature_type: igs
region: Genome
length_bp: 915
variable_sites: 27
pi: 0.00697
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
HRV17_pgt008-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	1034-1079 bp	1.0	67.8
HRV17_pgt008-rpoB_p2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	993-1038 bp	1.0	67.4
HRV17_pgt008-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1024-1069 bp	1.0	67.1
HRV17_pgt008-rpoB_p4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1029-1074 bp	1.0	67.0
HRV17_pgt008-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	1024-1069 bp	1.0	66.5