Hotspot

petN-psbM

Genome · 10249-10731 · 483 bp

Back to Sonchus

Pi 0.0115

MarkerSeek score 58.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 483
variable_sites: 20
pi: 0.0115
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.2	55.6	641-649 bp	1.0	63.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.2	56.4	641-649 bp	1.0	61.6
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	50.0	40.0	57.1	55.6	642-650 bp	1.0	57.3
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.9	55.6	633-641 bp	1.0	57.3
petN-psbM_p5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	47.6	40.0	58.0	55.6	627-635 bp	1.0	56.6