Hotspot

HRV17_pgp043

Genome · 69671-138870 · 69200 bp

Back to Sonchus

Pi 0.00274

MarkerSeek score 44.20

Primer available yes

Label HRV17_pgp043

Feature Metadata

label_name: HRV17_pgp043
feature_type: gene
region: Genome
length_bp: 69200
variable_sites: 899
pi: 0.00274
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
HRV17_pgp043_p1	TTGTTCGATGAGCAGATCCA	TCTGGATCCAAAGAATCAGTCA	45.0	40.9	56.9	56.8	298-351 bp	1.0	53.9
HRV17_pgp043_p2	TGTTCGATGAGCAGATCCAT	TCTGGATCCAAAGAATCAGTCA	45.0	40.9	56.7	56.8	297-350 bp	1.0	52.1
HRV17_pgp043_p3	TTGTTCGATGAGCAGATCCA	TGGATCCAAAGAATCAGTCAAGA	45.0	39.1	56.9	57.5	296-349 bp	1.0	51.4
HRV17_pgp043_p4	TTGTTCGATGAGCAGATCCA	TCTGGATCCAAAGAATCAGTCAA	45.0	39.1	56.9	57.5	298-351 bp	1.0	51.3
HRV17_pgp043_p5	TTGTTCGATGAGCAGATCCA	AACCAACTCATCACTTCGTA	45.0	40.0	56.9	54.3	321-374 bp	1.0	49.8