Hotspot

ycf1

SSC · 123884-129229 · 5346 bp

Back to Solms-laubachia

Pi 0.0171

MarkerSeek score 64.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5346
variable_sites: 564
pi: 0.0171
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCTACGTCGTTTAGGGGA	TGAACCTGTACGTTTGTCTGT	50.0	42.9	57.2	57.5	147-222 bp	1.0	73.5
ycf1_p2	TTCTCTACGTCGTTTAGGGGA	TGAACCTGTACGTTTGTCTGT	47.6	42.9	57.9	57.5	148-223 bp	1.0	71.2
ycf1_p3	TCTCTACGTCGTTTAGGGGAT	TGAACCTGTACGTTTGTCTGT	47.6	42.9	57.7	57.5	147-222 bp	1.0	69.5
ycf1_p4	TCTCTACGTCGTTTAGGGGA	TTGAACCTGTACGTTTGTCTGT	50.0	40.9	57.2	58.1	148-223 bp	1.0	68.6
ycf1_p5	TTCTCTACGTCGTTTAGGGG	TGAACCTGTACGTTTGTCTGT	50.0	42.9	56.4	57.5	148-223 bp	1.0	67.0