Hotspot

ndhF-rpl32

SSC · 112540-113376 · 837 bp

Back to Solms-laubachia

Pi 0.0356

MarkerSeek score 80.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 837
variable_sites: 164
pi: 0.0356
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCTGTCGGAAAAAGT	ACGAATACGCTTTTTCGAGA	40.0	40.0	56.4	55.5	954-996 bp	0.809524	51.9
ndhF-rpl32_p2	CTGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGA	47.6	40.0	57.6	55.5	906-948 bp	0.761905	51.3
ndhF-rpl32_p3	TTTGTTGCTGTCGGAAAAAGT	ACGAATACGCTTTTTCGAGA	38.1	40.0	57.1	55.5	955-997 bp	0.809524	49.9
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGAT	47.6	38.1	57.6	56.1	906-948 bp	0.809524	49.7
ndhF-rpl32_p5	TGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGA	45.0	40.0	56.3	55.5	905-947 bp	0.761905	49.5