Hotspot

psaJ-rpl33

LSC · 66775-67206 · 432 bp

Back to Solms-laubachia

Pi 0.0338
MarkerSeek score 75.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
432
variable_sites
78
pi
0.0338
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACCCGTGACATTTTGTACCC GGGGTGTTATGCCGATTCTT 50.0 50.0 58.1 57.9 1083-1133 bp 1.0 90.8
psaJ-rpl33_p2 ACCCGTGACATTTTGTACCC TCCAATCGACTAGGGGTGTT 50.0 50.0 58.1 58.1 1095-1145 bp 1.0 90.8
psaJ-rpl33_p3 ACCCGTGACATTTTGTACCC TTCCAATCGACTAGGGGTGT 50.0 50.0 58.1 58.1 1096-1146 bp 1.0 90.8
psaJ-rpl33_p4 CCCGTGACATTTTGTACCCA GGGGTGTTATGCCGATTCTT 50.0 50.0 58.1 57.9 1082-1132 bp 1.0 90.8
psaJ-rpl33_p5 CCCGTGACATTTTGTACCCA TCCAATCGACTAGGGGTGTT 50.0 50.0 58.1 58.1 1094-1144 bp 1.0 90.8