Hotspot

petA-psbJ

LSC · 62618-63564 · 947 bp

Back to Solms-laubachia

Pi 0.0333

MarkerSeek score 90.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 947
variable_sites: 163
pi: 0.0333
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 90.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.4	58.1	1023-1108 bp	1.0	78.7
petA-psbJ_p2	TAGGATCGGTTGTTTTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.4	57.8	1024-1109 bp	1.0	78.0
petA-psbJ_p3	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1049-1134 bp	0.952381	78.0
petA-psbJ_p4	ACGTGTCCAAGGACTTTTGT	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.6	57.8	1050-1135 bp	0.952381	77.3
petA-psbJ_p5	TCCATTACGTGTCCAAGGAC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.2	58.1	1055-1140 bp	1.0	77.2