Hotspot

trnT-ACA-trnL-UUA

LSC · 46127-46950 · 824 bp

Back to Solms-laubachia

Pi 0.0288
MarkerSeek score 89.60
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
824
variable_sites
149
pi
0.0288
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 GCGATGCTCTAACCTCTGAG CTACCGATTCCGCCATATCC 55.0 55.0 57.9 57.7 689-993 bp 1.0 88.6
trnT-ACA-trnL-UUA_p2 TATGGAAAAGCCGGCTATCG CTACCGATTCCGCCATATCC 50.0 55.0 57.8 57.7 740-1044 bp 1.0 88.3
trnT-ACA-trnL-UUA_p3 GGAATCGAACCGATGACCAT CTACCGATTCCGCCATATCC 50.0 55.0 57.8 57.7 721-1025 bp 1.0 87.7
trnT-ACA-trnL-UUA_p4 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 730-1034 bp 1.0 86.9
trnT-ACA-trnL-UUA_p5 TATGGAAAAGCCGGCTATCG GGTTTCCATACCAAGGCTCA 50.0 50.0 57.8 57.5 781-1085 bp 1.0 86.6