Hotspot

psbZ-trnG-GGC

LSC · 35806-36316 · 511 bp

Back to Solms-laubachia

Pi 0.0251

MarkerSeek score 84.90

Primer available yes

Label psbZ-trnG-GGC

Feature Metadata

label_name: psbZ-trnG-GGC
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 73
pi: 0.0251
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GGC_p1	TGTATTTGCGGCTCCTGATG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	602-708 bp	1.0	88.0
psbZ-trnG-GGC_p2	TTGTATTTGCGGCTCCTGAT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.6	57.8	603-709 bp	1.0	87.3
psbZ-trnG-GGC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	590-696 bp	0.952381	86.9
psbZ-trnG-GGC_p4	GTTGTATTTGCGGCTCCTGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.6	57.8	604-710 bp	1.0	86.2
psbZ-trnG-GGC_p5	CCTGATGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.9	57.8	589-695 bp	1.0	82.2