Hotspot

trnE-GAA-trnT-ACC

LSC · 30380-31225 · 846 bp

Back to Solms-laubachia

Pi 0.0306

MarkerSeek score 80.40

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 158
pi: 0.0306
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	AAGAGAGACGTCCTGAACCA	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	561-943 bp	1.0	91.9
trnE-GAA-trnT-ACC_p2	AAGAGAGACGTCCTGAACCA	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	540-922 bp	0.952381	89.6
trnE-GAA-trnT-ACC_p3	GCCTCCTTGAAAGAGAGACG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	946-953 bp	0.095238	44.3
trnE-GAA-trnT-ACC_p4	GCCTCCTTGAAAGAGAGACG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	925-932 bp	0.095238	43.9
trnE-GAA-trnT-ACC_p5	GCCTCCTTGAAAGAGAGACG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.0	58.1	922-929 bp	0.095238	43.7