Hotspot

petN-psbM

LSC · 27983-28515 · 533 bp

Back to Solms-laubachia

Pi 0.0343

MarkerSeek score 84.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 104
pi: 0.0343
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTCTCGTAGTGTGGGGAAGA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	641-668 bp	1.0	74.9
petN-psbM_p2	CTCTCTCGTAGTGTGGGGAA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	643-670 bp	1.0	74.9
petN-psbM_p3	TCTCTCGTAGTGTGGGGAAG	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	642-669 bp	1.0	74.9
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.0	57.0	638-665 bp	0.952381	74.3
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.9	57.0	637-664 bp	0.952381	73.7