Hotspot

trnC-UGC-petN

LSC · 27206-27892 · 687 bp

Back to Solms-laubachia

Pi 0.0268

MarkerSeek score 80.20

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 97
pi: 0.0268
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	681-855 bp	0.952381	89.4
trnC-UGC-petN_p2	GTTTCTATCGTTTTGGCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	737-911 bp	0.952381	89.2
trnC-UGC-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	682-856 bp	0.952381	88.8
trnC-UGC-petN_p4	GTTTCTATCGTTTTGGCGGC	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	738-912 bp	0.952381	88.6
trnC-UGC-petN_p5	TTTCCCCAGTTCAAATCCGG	TCTAGAGGCCACTTCTTCCC	50.0	55.0	58.1	58.1	691-865 bp	0.952381	88.4