Hotspot

rpoB-trnC-UGC

LSC · 26029-27134 · 1106 bp

Back to Solms-laubachia

Pi 0.0189

MarkerSeek score 85.10

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 143
pi: 0.0189
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1174-1238 bp	1.0	90.2
rpoB-trnC-UGC_p2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1156-1220 bp	1.0	88.0
rpoB-trnC-UGC_p3	GTTCCCTCTTTTCCATCCCC	TCAAGTCTGTTGTTGAGGCG	55.0	50.0	57.9	58.4	1198-1262 bp	1.0	87.5
rpoB-trnC-UGC_p4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.1	58.1	1175-1239 bp	1.0	84.1
rpoB-trnC-UGC_p5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	55.0	52.6	57.9	58.0	1196-1260 bp	1.0	82.8