Hotspot

trnK-AAA-rps16

LSC · 4394-4994 · 601 bp

Back to Solms-laubachia

Pi 0.0298

MarkerSeek score 90.60

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 103
pi: 0.0298
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 90.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	AAAGCCGAGTACTCTACCGT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	723-796 bp	1.0	87.5
trnK-AAA-rps16_p2	AAGCCGAGTACTCTACCGTT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	722-795 bp	1.0	87.5
trnK-AAA-rps16_p3	TACCGTTGAGTTAGCAACCC	CGCTCAACCAACAAGAACAG	50.0	50.0	57.5	57.6	709-782 bp	1.0	85.2
trnK-AAA-rps16_p4	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	734-807 bp	1.0	84.3
trnK-AAA-rps16_p5	TCGCACTTAAAAGCCGAGTA	CGCTCAACCAACAAGAACAG	45.0	50.0	57.3	57.6	732-805 bp	1.0	83.1