Hotspot

trnT-GGU

LSC · 31227-31294 · 68 bp

Back to Solidago

Pi 0.269

MarkerSeek score 72.10

Primer available yes

Label trnT-GGU

Feature Metadata

label_name: trnT-GGU
feature_type: tRNA
region: LSC
length_bp: 68
variable_sites: 14
pi: 0.269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU_p1	TGGATATGTGTAGACGGGCT	TGTGGAAGAACGAAATGCCA	50.0	45.0	57.9	58.0	768-932 bp	1.0	87.2
trnT-GGU_p2	AGGGGCTTCTGGATATGTGT	TGTGGAAGAACGAAATGCCA	50.0	45.0	58.1	58.0	777-941 bp	1.0	87.0
trnT-GGU_p3	ATCTTTTGTCGAGGGGCTTC	TGTGGAAGAACGAAATGCCA	50.0	45.0	57.9	58.0	788-952 bp	1.0	86.8
trnT-GGU_p4	GATCTTTTGTCGAGGGGCTT	TGTGGAAGAACGAAATGCCA	50.0	45.0	57.9	58.0	789-953 bp	1.0	86.8
trnT-GGU_p5	GTGACCCGTTCAGGAATTCA	TGTGGAAGAACGAAATGCCA	50.0	45.0	57.8	58.0	655 bp	0.5	58.4