Hotspot

rps19

LSC · 84699-84977 · 279 bp

Back to Solidago

Pi 0.00717

MarkerSeek score 50.40

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 2
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGTCATAAAGTTCGCCTCCT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	56.6	58.1	444-449 bp	1.0	68.0
rps19_p2	TGTCATAAAGTTCGCCTCCT	ATTTGATTCTTCGTCGCCGT	45.0	45.0	56.6	58.3	446-451 bp	1.0	66.6
rps19_p3	TTGTCATAAAGTTCGCCTCCT	TTGATTCTTCGTCGCCGTAG	42.9	50.0	57.3	58.1	445-450 bp	1.0	66.0
rps19_p4	TGTCATAAAGTTCGCCTCCT	TTTGATTCTTCGTCGCCGTA	45.0	45.0	56.6	57.6	445-450 bp	1.0	66.0
rps19_p5	GTTGTCATAAAGTTCGCCTCC	TTGATTCTTCGTCGCCGTAG	47.6	50.0	57.3	58.1	446-451 bp	1.0	65.7