Hotspot

trnL-UAG-rpl32

SSC · 123990-124825 · 836 bp

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Pi 0.00726
MarkerSeek score 64.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
836
variable_sites
6
pi
0.00726
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GAAAGGATATTGGGCGTCGT 50.0 50.0 58.0 58.1 966-985 bp 1.0 80.8
trnL-UAG-rpl32_p2 AGCGTGTCTACCGATTTCAC TGAAAGGATATTGGGCGTCG 50.0 50.0 58.0 58.1 967-986 bp 1.0 80.8
trnL-UAG-rpl32_p3 CAGCGTGTCTACCGATTTCA GAAAGGATATTGGGCGTCGT 50.0 50.0 58.0 58.1 967-986 bp 1.0 80.8
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA TGAAAGGATATTGGGCGTCG 50.0 50.0 58.0 58.1 968-987 bp 1.0 80.8
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GAAAGGATATTGGGCGTCGT 50.0 50.0 57.9 58.1 976-995 bp 1.0 79.9