Hotspot

ycf1-rps15

SSC · 114447-114899 · 453 bp

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Pi 0
MarkerSeek score 59.50
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
SSC
length_bp
453
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 GGGAGCCGTTTGAGTATGTT AGTCTTCGTATCTTGTTGGTGA 50.0 40.9 57.9 57.1 590-608 bp 1.0 58.6
ycf1-rps15_p2 ATGGGAGCCGTTTGAGTATG AGTCTTCGTATCTTGTTGGTGA 50.0 40.9 57.7 57.1 592-610 bp 1.0 56.8
ycf1-rps15_p3 GGGAGCCGTTTGAGTATGTT AGTCTTCGTATCTTGTTGGTGAA 50.0 39.1 57.9 57.8 590-608 bp 1.0 55.9
ycf1-rps15_p4 GGGAGCCGTTTGAGTATGTT AAGTCTTCGTATCTTGTTGGTGA 50.0 39.1 57.9 57.8 591-609 bp 1.0 55.8
ycf1-rps15_p5 GGGAGCCGTTTGAGTATGTT GTCTTCGTATCTTGTTGGTGA 50.0 42.9 57.9 55.7 589-607 bp 1.0 54.8