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psbT-psbN

LSC · 74711-74783 · 73 bp

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Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACGATCGAATCTATGGAAGCA	TCGAGGAACATGGGGACTAA	42.9	50.0	57.0	57.8	207 bp	1.0	62.4
psbT-psbN_p2	ACGATCGAATCTATGGAAGCA	TTCGAGGAACATGGGGACTA	42.9	50.0	57.0	57.8	208 bp	1.0	62.4
psbT-psbN_p3	ACGATCGAATCTATGGAAGCA	GATCCCTTCGAGGAACATGG	42.9	55.0	57.0	57.8	214 bp	1.0	62.3
psbT-psbN_p4	ACGATCGAATCTATGGAAGCA	GGGTACGCCTTATATACCGC	42.9	55.0	57.0	57.7	262 bp	1.0	62.0
psbT-psbN_p5	ACCACGATCGAATCTATGGA	TCGAGGAACATGGGGACTAA	45.0	50.0	55.8	57.8	210 bp	1.0	61.2