Hotspot

ycf3-trnS-GCU

LSC · 44451-45305 · 855 bp

Back to Solidago

Pi 0

MarkerSeek score 60.00

Primer available yes

Label ycf3-trnS-GCU

Feature Metadata

label_name: ycf3-trnS-GCU
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GCU_p1	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1016-1027 bp	1.0	66.4
ycf3-trnS-GCU_p2	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1017-1028 bp	1.0	66.4
ycf3-trnS-GCU_p3	TTCTCCTGAAGTTGTCGGAA	CCTCGGTAAGCAAAAGCCTA	45.0	50.0	56.4	57.6	1006-1017 bp	1.0	65.0
ycf3-trnS-GCU_p4	TTTCTCCTGAAGTTGTCGGA	CCTCGGTAAGCAAAAGCCTA	45.0	50.0	56.4	57.6	1007-1018 bp	1.0	65.0
ycf3-trnS-GCU_p5	TTCTCCTGAAGTTGTCGGAA	GATTCGAACCCTCGGTAAGC	45.0	55.0	56.4	58.4	1015-1026 bp	1.0	64.7