Hotspot

trnG-UCC-trnT-GGU

LSC · 30865-31226 · 362 bp

Back to Solidago

Pi 0.128
MarkerSeek score 67.20
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
362
variable_sites
29
pi
0.128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.63
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 TGGTTTCATTCGGCTCCTTT TCCCCTATCGGATCTGAACC 45.0 55.0 57.7 58.0 971-1122 bp 1.0 84.9
trnG-UCC-trnT-GGU_p2 TTGGTTTCATTCGGCTCCTT TCCCCTATCGGATCTGAACC 45.0 55.0 57.7 58.0 972-1123 bp 1.0 84.9
trnG-UCC-trnT-GGU_p3 TTTGGTTTCATTCGGCTCCT TCCCCTATCGGATCTGAACC 45.0 55.0 57.7 58.0 973-1124 bp 1.0 84.9
trnG-UCC-trnT-GGU_p4 TGGTTTCATTCGGCTCCTTT TGTGGAAGAACGAAATGCCA 45.0 45.0 57.7 58.0 1509-1673 bp 1.0 83.7
trnG-UCC-trnT-GGU_p5 TTGGTTTCATTCGGCTCCTT TGTGGAAGAACGAAATGCCA 45.0 45.0 57.7 58.0 1510-1674 bp 1.0 83.7