Hotspot

ndhA

SSC · 121149-123398 · 2250 bp

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Pi 0.0171

MarkerSeek score 70.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 520
pi: 0.0171
unique_haplotype_count: 157
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAGACAAGTCGTGAATCTAGG	TGGGAGAAGTTGATCGTTGA	45.5	45.0	57.3	56.5	2259-2338 bp	0.979695	57.5
ndhA_p2	TGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	55.2	56.5	2263-2342 bp	0.979695	56.6
ndhA_p3	TTGTTTGAGACAAGTCGTGA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	55.2	56.5	2264-2343 bp	0.979695	56.6
ndhA_p4	TGAGACAAGTCGTGAATCTAGG	TGGGAGAAGTTGATCGTTGAA	45.5	42.9	57.3	57.2	2259-2338 bp	0.979695	55.5
ndhA_p5	GAGACAAGTCGTGAATCTAGGA	TGGGAGAAGTTGATCGTTGA	45.5	45.0	57.0	56.5	2258-2337 bp	0.979695	55.4