Hotspot

cemA

LSC · 63064-63753 · 690 bp

Back to Solanum

Pi 0.0478

MarkerSeek score 64.20

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 159
pi: 0.0478
unique_haplotype_count: 71
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GGCAAACCATTTCGAAAGCA	GTTCCCTAGCCACGATTCTG	45.0	55.0	57.9	58.1	1253-1479 bp	0.817259	83.0
cemA_p2	GGCAAACCATTTCGAAAGCA	ACTTGAAATGGACGTCCGAG	45.0	50.0	57.9	57.9	1411-1637 bp	0.812183	82.7
cemA_p3	GGCAAACCATTTCGAAAGCA	CTTGAAATGGACGTCCGAGT	45.0	50.0	57.9	57.9	1410-1636 bp	0.812183	82.7
cemA_p4	GGCAAACCATTTCGAAAGCA	TGGAACCTCAATTTCCACGG	45.0	50.0	57.9	58.1	1522-1748 bp	0.80203	82.1
cemA_p5	TTCATCTCAGACGGCAAACC	GTTCCCTAGCCACGATTCTG	50.0	55.0	58.2	58.1	1265-1485 bp	0.695431	77.8