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ndhF-rpl32

SSC · 113742-114526 · 785 bp

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Pi 0.0349

MarkerSeek score 76.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 785
variable_sites: 325
pi: 0.0349
unique_haplotype_count: 160
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGAAAGGTATGATCCACGCA	TGATGTCGAAGTGCGTTTTT	45.0	40.0	56.9	56.6	655-894 bp	0.974619	71.1
ndhF-rpl32_p2	TGAAAGGTATGATCCACGCA	TTGATGTCGAAGTGCGTTTT	45.0	40.0	56.9	56.6	656-895 bp	0.974619	71.1
ndhF-rpl32_p3	TGAAAGGTATGATCCACGCA	TTTGATGTCGAAGTGCGTTT	45.0	40.0	56.9	56.6	657-896 bp	0.974619	71.1
ndhF-rpl32_p4	TGAAAGGTATGATCCACGCA	TTTTGATGTCGAAGTGCGTT	45.0	40.0	56.9	56.6	658-897 bp	0.969543	70.9
ndhF-rpl32_p5	TGAAAGGTATGATCCACGCA	TTTTTGATGTCGAAGTGCGT	45.0	40.0	56.9	56.6	659-898 bp	0.969543	70.9