Hotspot

psbE-petL

LSC · 66794-67781 · 988 bp

Back to Solanum

Pi 0.0167

MarkerSeek score 68.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 273
pi: 0.0167
unique_haplotype_count: 129
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCCGCTAATAGAAAACCGA	45.0	45.0	58.0	57.0	1092-1186 bp	0.994924	83.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCCGCTAATAGAAAACCGA	50.0	45.0	57.9	57.0	1081-1175 bp	0.994924	83.6
psbE-petL_p3	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.0	57.1	1138-1232 bp	0.979695	83.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1127-1221 bp	0.979695	83.2
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1045-1139 bp	0.979695	82.1