Hotspot

atpB-rbcL

LSC · 55941-56752 · 812 bp

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Pi 0.0209

MarkerSeek score 65.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 229
pi: 0.0209
unique_haplotype_count: 111
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	816-941 bp	0.994924	82.9
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	815-940 bp	0.994924	82.7
atpB-rbcL_p3	GGAAACCCCAGAACCAGAAG	CCTTGGTTTGGTACTCAGGAG	55.0	52.4	58.1	57.9	864-989 bp	0.989848	82.4
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	CCTTGGTTTGGTACTCAGGA	55.0	50.0	58.1	56.8	864-989 bp	0.984772	80.7
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	TCCTTGGTTTGGTACTCAGG	55.0	50.0	58.1	56.8	865-990 bp	0.984772	80.7