Hotspot

ndhC-trnV-UAC

LSC · 51817-52918 · 1102 bp

Back to Solanum

Pi 0.0205

MarkerSeek score 66.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 335
pi: 0.0205
unique_haplotype_count: 134
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.9	57.8	917-1243 bp	0.994924	51.8
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.9	57.5	916-1242 bp	0.994924	51.8
ndhC-trnV-UAC_p3	ATCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	918-1244 bp	0.994924	51.8
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	917-1243 bp	0.994924	51.8
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	905-1231 bp	0.994924	51.8